Recombinant mRuby antibody (Biotin)
Quick Overview for Recombinant mRuby antibody (Biotin) (ABIN7272894)
Target
Antibody Type
Fragment
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Expression System
- E.coli
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Specificity
- Recognizes most common mRuby derivatives
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Purification
- Produced in: E.coli
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Immunogen
- sdAb anti-mRUBY is derived from an in-house developed single-domain antibody (sdAb) that recognizes mRuby and mRuby derivatives, which is a bright monomeric red fluorescent protein derived from Entacmaea quadricolor.
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anti-mRuby antibody (Atto 488)
Reactivity: Entacmaea quadricolor IF, ICC, FACS Host: Alpaca Monoclonal 1F8 Atto 488 single-domain Antibody (sdAb) Recombinant Antibody
anti-mRuby antibodyReactivity: Discosoma IF, IEM, IHC (fro), IHC (p), WB Host: Goat Polyclonal unconjugated
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Application Notes
- Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconsitute with 250 µL 50% glycerol for storage at -80 °C
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Buffer
- sdAbs are lyophilized from PBS pH 7.4.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
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- mRuby
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Background
- MRuby is a bright monomeric red fluorescent protein, which has more than 20 mutations compared to its wild-type predecessor (eqFP611) found in Entacmaea quadricolor . mRuby, mRuby2 & mRuby3 are all exited at 560 nm, with mRuby3 being the brightest.
Target
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